Trident Peptide Technologies: Performance, Healing & Longevity

Welcome to the cutting edge of wellness science.

Each Trident peptide is selected to target recovery, regeneration, and vitality — designed to amplify your body’s natural healing and performance systems.

This is your number one resource here https://www.peptideresearchinstitute.org/blank-4

Below is a quick guide if you dont want to use the link above

BPC-157 + TB-500 (The Healing Duo)

Category: Regeneration & Pain Recovery

How it works:

These two peptides complement each other to accelerate tissue repair.

  • BPC-157 supports healing of muscles, tendons, ligaments, and the gut lining.

  • TB-500 (Thymosin Beta-4) enhances cell migration, angiogenesis, and recovery from inflammation or injury.
    Mixing Ratio:

    • 20 mg vial — add 3 mL bacteriostatic water

    Suggested Dosage:

    • 1 IU min dail y- no max

    • Inject near injured muscle, joint, or abdominal area

    • Cycle 4–8 weeks for acute injuries or ongoing joint support. Can stay on long term.

    Benefits:

    • Accelerates healing of muscles, tendons, ligaments & connective tissue

    • Reduces pain and inflammation throughout the body

    • Enhances circulation and cellular regeneration

    • Supports gut healing and overall recovery response

    • Improves mobility and flexibility for athletic performance

    • Promotes faster recovery after training or injury

    Best Stacked With:

    Tesamorelin + Glutathione for advanced recovery and anti-inflammatory support
    Best for:

    Athletes, anyone recovering from injury, chronic pain, or surgery.

    Mixing tip: Combine in the same solution or alternate daily; both dissolve easily in bacteriostatic water.

    Stack synergy: Excellent with NAD⁺ or Pinealon for systemic recovery.

Tesamorelin 5mg

Category: Growth Hormone Releasing Peptide

Mixing Ratio:

• 5 mg vial — add 2 mL bacteriostatic water (5 mg/mL)

Suggested Dosage:

• .05 daily (AM or PM fasted, subcutaneous in the abdomen)

• Best injected on an empty stomach before breakfast or workout

• Cycle: 3–6 months for optimal results

Benefits:

• Stimulates natural growth hormone (GH) production

• Accelerates fat loss — especially in the abdomen and visceral areas

• Enhances muscle growth, strength, and performance recovery

• Supports skin, hair, and connective tissue repair

• Boosts sleep quality, energy, and mood through GH balance

• Improves cognitive clarity and long-term body composition

Best Stacked With:

AOD-9604 + Retatrutide or Semaglutide for advanced fat reduction and physique refinement

How it works:

A potent secretagogue that stimulates natural GH release, reducing visceral fat while maintaining lean muscle.

Benefits:

  • Decreases belly fat

  • Improves sleep and skin quality

  • Enhances muscle tone and metabolism

    Stack synergy: Combine with AOD-9604 or IGF-1 LR3 for advanced body recomposition.

    KPV — 10 mg (Educational Math)

    Example Reconstitution Options

    Option A: Add 2 mL bacteriostatic water

    • Concentration: 5 mg/mL

    • 10 units = 0.5 mg

    • 20 units = 1 mg

    Option B: Add 1 mL bacteriostatic water (more concentrated)

    • Concentration: 10 mg/mL

    • 10 units = 1 mg

    • 5 units = 0.5 mg

    Cerebrolysin — 60 mg (Educational Math)

Cerebrolysin is a neurotrophic peptide complex often discussed in clinical/research contexts. The information below is math only, not usage instruction.

Example Reconstitution Options

Option A: Add 6 mL sterile water

  • Concentration: 10 mg/mL

  • 10 units = 1 mg

  • 50 units = 5 mg

  • 100 units = 10 mg

Option B: Add 3 mL sterile water (more concentrated)

  • Concentration: 20 mg/mL

  • 10 units = 2 mg

Semax — 5 mg (Educational Math)

Semax is often discussed in education circles in intranasal formats.

Example Intranasal Mixing Math

Add 5 mL sterile saline

  • Concentration: 1 mg/mL

Spray note: nasal spray bottles vary. Many deliver ~0.1 mL per spray (varies by bottle).

  • If ~0.1 mL per spray: ~100 mcg per spray

DSIP — 5 mg (Educational Math)

Example Reconstitution Options

Option A: Add 2 mL bacteriostatic water

  • Concentration: 2.5 mg/mL

  • 10 units = 0.25 mg (250 mcg)

  • 20 units = 0.5 mg (500 mcg)

Option B: Add 1 mL bacteriostatic water (more concentrated)

  • Concentration: 5 mg/mL

  • 10 units = 0.5 mg

  • 20 units = 1 mg

LL-37 — 5 mg (Educational Math)

Example Reconstitution Options

Option A: Add 2 mL bacteriostatic water

  • Concentration: 2.5 mg/mL

  • 10 units = 0.25 mg

  • 20 units = 0.5 mg

Option B: Add 1 mL bacteriostatic water (more concentrated)

  • Concentration: 5 mg/mL

  • 10 units = 0.5 mg

Selank — 11 mg (Educational Math)

Selank is often discussed in education circles in intranasal formats.

Example Intranasal Mixing Math

Option A: Add 11 mL sterile saline

  • Concentration: 1 mg/mL

  • If ~0.1 mL per spray (varies by bottle): ~100 mcg per sprayAOD-9604

Category: Fat Metabolism Accelerator

Mixing Ratio:

• 5 mg vial — add 2 mL bacteriostatic water (2.5 mg/mL)

• Example: 250 mcg = 0.1 mL (10 units on an insulin syringe)

Suggested Dosage:

• 250–500 mcg daily (subcutaneous in abdomen or thigh)

• Best injected morning or pre-cardio on an empty stomach

• Cycle 8–12 weeks for fat loss and metabolic support

Benefits:

• Stimulates fat breakdown without affecting blood sugar or appetite

• Targets stubborn belly and thigh fat deposits

• Supports cartilage repair and joint health

• Improves recovery and metabolic efficiency

• Synergistic with Tesamorelin for GH release and recomposition

IGF-1 LR3

Mixing Ratio:

• 1 mg vial — add 2 mL bacteriostatic water (0.5 mg/mL)

• Example: 50 mcg = 0.1 mL (10 units on an insulin syringe)

Suggested Dosage:

• .05 daily (subcutaneous or intramuscular post-workout) Then eat carbs.

• Cycle 4–6 weeks with a 2–4 week break between rounds

• Best taken on training days to enhance muscle growth and recovery

Benefits:

• Promotes lean muscle growth and enhanced recovery

• Increases strength, power, and endurance capacity

• Improves muscle cell repair and protein synthesis

• Accelerates healing of tendons and connective tissue

• Enhances fat metabolism and nutrient partitioning

Best Stacked With:

Tesamorelin + BPC-157/TB-500 for muscle growth, healing, and performance optimization

Category: Muscle Growth & Repair

How it works:

Insulin-like Growth Factor-1 LR3 extends IGF’s half-life, promoting muscle cell growth and recovery.

Benefits:

  • Promotes new muscle fiber development

  • Speeds recovery between sessions

  • Improves insulin sensitivity and nutrient uptake

    Stack synergy: Works beautifully with Tesamorelin or Follistatin.

Follistatin 344

Mixing Ratio:

• 1 mg vial — add 2 mL bacteriostatic water (0.5 mg/mL)

• Example: 100 mcg = 0.2 mL (20 units on an insulin syringe)

Suggested Dosage:

• 100–200 mcg daily (subcutaneous or intramuscular)

• Typical cycle: 10–20 days followed by 4–6 weeks off

• Best used post-workout for muscle regeneration and growth signaling

Benefits:

• Inhibits myostatin — unlocking faster muscle growth potential

• Promotes lean tissue gain while reducing fat accumulation

• Accelerates recovery and cellular repair after intense training

• Enhances strength, endurance, and body recomposition

• Synergistic with IGF-1 LR3 for muscle density and tone

Best Stacked With:

IGF-1 LR3 + Tesamorelin for advanced muscle growth and recovery synergy

Category: Myostatin Inhibitor

How it works:

Suppresses the myostatin protein that limits muscle growth, unlocking new levels of lean mass potential.

Benefits:

  • Increases muscle size and strength

  • Accelerates recovery and fat loss

  • Ideal for recomposition or advanced training phases

    Stack synergy: Combine with IGF-1 LR3 or Tesamorelin for synergistic effect.

Pinealon

Mixing Ratio:

• 10 mg vial — add 2 mL bacteriostatic water (5 mg/mL)

• Example: 200 mcg = 0.04 mL (4 units on an insulin syringe)

Suggested Dosage: Take midday

• 100–200 mcg daily (subcutaneous or intranasal)

• Cycle 20–30 days for neurological rejuvenation and clarity

Benefits:

• Supports brain regeneration and mitochondrial health

• Enhances focus, learning, and emotional balance

• Promotes deep rest, vivid dreams, and spiritual awareness

• Protects neurons from oxidative stress and aging

• Elevates energy, mood, and intuitive connection

Best Stacked With:

Epithalon + NAD⁺ for brain longevity, spiritual clarity, and light-body activation

Category: Cognitive & Longevity Support

How it works:

A tri-peptide that targets the pineal gland and brain cells to restore RNA/DNA synthesis and neuroprotection.

Benefits:

  • Improves focus, mental clarity, and sleep

  • Reduces oxidative stress in neurons

  • Supports mood and long-term brain function

    Stack synergy: Great with NAD⁺ or SS-31 for cellular rejuvenation.

  • 💉 Semaglutide

    Mixing Ratio:

    • 5 mg vial — add 2 mL bacteriostatic water (2.5 mg/mL)

    • Example: 250 mcg = 0.1 mL (10 units on an insulin syringe)

    Suggested Dosage:

    • Start: 250 mcg once weekly

    • Week 3–4: 500 mcg once weekly

    • Week 5+: 750–1,000 mcg weekly (maximum therapeutic range)

    • Administer subcutaneously in the abdomen or thigh

    Benefits:

    • GLP-1 receptor agonist that promotes steady, sustainable fat loss

    • Significantly reduces appetite and cravings for sugar or alcohol

    • Stabilizes blood sugar and improves insulin sensitivity

    • Enhances energy, focus, and metabolic control throughout the day

    • May reduce inflammation and improve cardiovascular health

    • Helps preserve lean muscle while reducing visceral fat

    Best Stacked With:

    AOD-9604 + Tesamorelin for advanced body-recomposition and metabolic synergy

SS-31 (Elamipretide)

How to Mix and Dose SS-31

SS-31, also known as Elamipretide, is a synthetic peptide designed to target and protect mitochondria, promoting cellular health and energy. Proper mixing and dosing are essential for effectiveness and safety.

Mixing SS-31

10mg add 2 ml water

Dosing SS-31

Take a minium of .10 daily

Category: Mitochondrial Repair

How it works:

Targets and protects mitochondria — your body’s energy generators — from oxidative damage.

Benefits:

  • Enhances cellular energy (ATP production)

  • Improves heart and muscle endurance

  • Combats fatigue and aging signs

    Stack synergy: Combine with NAD⁺ and Pinealon for anti-aging and vitality.

NAD⁺ 500mg(Nicotinamide Adenine Dinucleotide)

Add 5Mg water

Start at 50 units

Category: Longevity & Energy

How it works:

Boosts cellular energy metabolism and DNA repair pathways.

Benefits:

  • Cellular detox and repair

  • Improves cognitive functio

  • Enhances energy and endurance

    Stack synergy: Perfect base for any protocol — enhances all other peptide effects.

MOTS-C

Category: Metabolic Rebalancer

How it works:

A mitochondrial-derived peptide that regulates insulin sensitivity and fat oxidation.

Benefits:

  • Improves metabolism and endurance

  • Reduces fat storag

  • Supports stable blood sugar levels

    Stack synergy: Combine with AOD-9604 and Tesamorelin for peak metabolic output.

GHK-Cu

100mg add 2ml water

Take a min of .10 daily or as needed

Category: Skin, Hair & Tissue Renewal

How it works:

A naturally occurring copper peptide that rejuvenates skin and hair while supporting collagen and elastin production.

Benefits:

  • Reduces wrinkles and fine lines

  • Stimulates hair growth

  • Speeds wound and scar healing

    Stack synergy: Works synergistically with BPC-157 for tissue repair or topical skin renewal protocols.

SNAP-8 (Acetyl Octapeptide-3) — 10 mg Bottle

Mixing & Usage (Educational Overview Only)

What SNAP-8 Is

SNAP-8 is a topical cosmetic peptide used in skincare education for its ability to:

  • Support the appearance of smoother skin

  • Reduce the look of expression lines

  • Promote a more relaxed, refreshed look

🚫 Not injectable
Topical use only

Reconstitution / Mixing (10 mg Vial)

Option 1: Add 1 mL sterile water

  • Total peptide: 10 mg

  • Concentration: 10 mg per mL

This creates a liquid concentrate that can be mixed into:

  • Face serums

  • Creams or lotions

  • Hyaluronic acid bases🧬 Retatrutide

Mixing Ratio:

• 5 mg vial — add 2 mL bacteriostatic water (2.5 mg/mL)

• Example: 250 mcg = 0.1 mL (10 units on an insulin syringe)

Suggested Dosage:

• Start: 250 mcg once weekly

• Increase gradually to 500–750 mcg weekly, as tolerated

• Administer subcutaneously in the abdomen or thigh

Benefits:

• Powerful multi-receptor GLP-1/GIP/glucagon agonist

• Accelerates fat loss and improves insulin sensitivity

• Suppresses appetite and cravings for sweets or alcohol

• Enhances energy, focus, and motivation through stable blood sugar

• Preserves lean muscle mass while promoting metabolic balance

• May improve liver fat, cholesterol, and inflammation markers

Best Stacked With:

Tesamorelin + AOD-9604 for advanced fat loss and body recomposition

✨ Glutathione

Mixing Ratio:

• 600 mg vial — add 2 mL bacteriostatic water (300 mg/mL)

• Example: 200 mg = 0.66 mL (≈ 6–7 units on an insulin syringe)

Suggested Dosage:

• 200–600 mg, 2–3× weekly (IM or subcutaneous)

• Can also be taken orally or IV, depending on preference

Benefits:

• Master antioxidant — protects cells from oxidative stress

• Promotes bright, clear, glowing skin and reduces pigmentation

• Detoxifies liver and supports immune resilience

• Aids recovery from inflammation, illness, or heavy training

• Supports brain clarity, energy, and anti-aging pathways

• Pairs beautifully with NAD⁺, BPC-157, or Pinealon for regeneration

Best Stacked With:

GHK-Cu + Pinealon for cellular renewal and skin rejuvenation

Building Your Stack

🔹 Hair Growth Stack

Peptides: GHK-Cu • BPC-157 • TB-500

Mixing Ratios & Dosages

• GHK-Cu — 5 mg vial: add 2 mL BAC water (2.5 mg/mL) → Inject 100 mcg daily subQ near scalp or topically in serum form

• BPC-157 — 5 mg vial: add 2 mL BAC water (2.5 mg/mL) → 250 mcg 1-2× daily subQ near injury or scalp line

• TB-500 — 5 mg vial: add 2 mL BAC water → 2-5 mg weekly for tissue healing and circulation support

Benefits:

• Stimulates new hair growth and reduces shedding

• Improves scalp blood flow and follicle strength

• Repairs micro-damage and supports collagen formation

• Restores shinier, thicker hair texture

Best Stacked With: GHK-Cu topical + Glutathione for detox and shine

💎 Skin Regeneration Stack

Peptides: GHK-Cu • Epithalon • Thymosin Beta-4

Mixing Ratios & Dosages

• GHK-Cu — as above

• Epithalon — 10 mg vial: add 2 mL BAC water (5 mg/mL) → 2-10 mg nightly for 20 days every 3-6 months

• TB-500 — as above

Benefits:

• Boosts collagen and elasticity for firm skin

• Reduces fine lines and photo-damage

• Accelerates wound healing and recovery from procedures

• Restores cellular youth and radiance

Best Stacked With: Glutathione + NAD⁺ for advanced skin clarity and detox

🌙 Mental Health & Sleep Stack

Peptides: Selank • DSIP • Pinealon

Mixing Ratios & Dosages

• Selank — 5 mg vial: add 2 mL BAC water (2.5 mg/mL) → 250-500 mcg daily (subQ or nasal spray)

• DSIP — 5 mg vial: add 2 mL BAC water → 100-300 mcg before bed

• Pinealon — 10 mg vial: add 2 mL BAC water → 100-200 mcg daily AM or PM

Benefits:

• Lowers anxiety and stress hormones

• Promotes deep, restorative sleep and lucid dreams

• Enhances focus, memory, and emotional stability

• Regulates neurotransmitters for mood balance

Best Stacked With: NAD⁺ or Epithalon for brain longevity and clarity

🔮 Spiritual Awakening & Progression Stack

Peptides: Pinealon • Epithalon • NAD⁺

Mixing Ratios & Dosages

• Pinealon — as above

• Epithalon — as above

• NAD⁺ — 100 mg vial: add 2 mL BAC water (50 mg/mL) → 25-50 mg IM 2-3× weekly

Benefits:

• Sharpens intuition and mental clarity

• Supports mitochondrial energy and anti-aging

• Boosts DNA repair and cellular communication

• Promotes deep meditation and energetic alignment

Best Stacked With: Glutathione for energetic detox and light-body clarity